Mouse IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit

Product Specifications

Abbreviation

IP-10; CXCL10

UniProt

P17515

Cross Reactivity

This kit recognizes Mouse IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Mouse IP-10/CXCL10 and analogues was observed

Target

C7; IFI10; INP10; SCYB10; crg-2; gIP-10; mob-1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IP-10/CXCL10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IP-10/CXCL10. You can calculate the concentration of Mouse IP-10/CXCL10 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://jitc.bmj.com/content/10/6/e004381.abstract

Citation 02

https://www.sciencedirect.com/science/article/pii/S0144861722009377

Citation 03

https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btm2.10357

Citation 04

https://www.sciencedirect.com/science/article/pii/S0022202X21012379

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8511489/

Citation 06

https://link.springer.com/article/10.1186/s12575-022-00177-9

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.17236

Citation 08

https://link.springer.com/article/10.1007/s00011-021-01455-0

Citation 09

https://www.sciencedirect.com/science/article/pii/S1567576920317653

Citation 10

https://www.sciencedirect.com/science/article/pii/S1567576922001424

Citation 11

https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.14336

Citation 12

https://ar.iiarjournals.org/content/42/11/5205.abstract

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IP-10/CXCL10 concentrations in serum, plasma and other biological fluids.

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