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Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit

Product Specifications

Abbreviation

RANTES

UniProt

P13501

Cross Reactivity

This kit recognizes Human RANTES in samples.No significant cross-reactivity or interference between Human RANTES and analogues was observed

Target

CCL5; SCYA5; SISd; TCP228; D17S136E

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RANTES. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RANTES. You can calculate the concentration of Human RANTES in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Product Datasheet

https://789.bio/ea/j9w5at

Product MSDS

https://789.bio/eb/qDCaHK

Citation 01

https://clincancerres.aacrjournals.org/content/25/6/1957.abstract

Citation 02

https://www.nature.com/articles/srep23103

Citation 03

https://www.frontiersin.org/articles/10.3389/fphys.2022.856088/full?utm_source=dlvr.it&utm_medium=twitter

Citation 04

https://www.mdpi.com/1131626

Citation 05

https://www.sciencedirect.com/science/article/pii/S0753332218384737

Citation 06

https://www.sciencedirect.com/science/article/pii/S0001706X18302559

Citation 07

https://www.spandidos-publications.com/10.3892/mmr.2017.6969?text=abstract

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human RANTES concentrations in serum, plasma and other biological fluids.

Available Sizes

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