Human POSTN/OSF-2 (Periostin) ELISA Kit

Product Specifications

Abbreviation

POSTN; OSF-2

UniProt

Q15063

Cross Reactivity

This kit recognizes Human POSTN/OSF-2 in samples.No significant cross-reactivity or interference between Human POSTN/OSF-2 and analogues was observed

Target

OSF2; PDLPOSTN; PN; RP11-412K4.1; Osteoblast Specific Factor 2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Signal transduction; Tags and Cell Markers

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human POSTN/OSF-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human POSTN/OSF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human POSTN/OSF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human POSTN/OSF-2. You can calculate the concentration of Human POSTN/OSF-2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.09 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1007/s12020-022-03027-6

Citation 02

https://link.springer.com/article/10.1007/s00784-022-04681-w

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/ijcp.14728

Citation 04

https://link.springer.com/article/10.1007/s10157-019-01744-3

Citation 05

https://www.tandfonline.com/doi/abs/10.1080/20469047.2019.1682330

Citation 06

https://www.sciencedirect.com/science/article/pii/S2213398421001780

Citation 07

https://www.degruyter.com/document/doi/10.1515/tjb-2022-0081/html

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human POSTN/OSF-2 concentrations in serum, plasma and other biological fluids.

Available Sizes

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