Human IL-6 (Interleukin 6) ELISA Kit

Product Specifications

Abbreviation

IL-6

UniProt

P05231

Cross Reactivity

This kit recognizes Human IL-6 in samples.No significant cross-reactivity or interference between Human IL-6 and analogues was observed

Target

IL6; BSF2; HGF; HSF; IFNB2; CDF

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Immunology; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided inthis kit has been pre-coated with an antibody specific to Human IL-6. Samples (orStandards) are added to the micro ELISA plate wells and combined with the specificantibody. Then a biotinylated detection antibody specific for Human IL-6 and AvidinHorseradish Peroxidase (HRP) conjugate are added successively to each micro plate welland incubated. Free components are washed away. The substrate solution is added to eachwell. Only those wells that contain Human IL-6, biotinylated detection antibody andAvidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction isterminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value isproportional to the concentration of Human IL-6. You can calculate the concentration ofHuman IL-6 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-021-01369-9

Citation 02

https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.31056

Citation 03

https://pubs.acs.org/doi/abs/10.1021/acsnano.6b06658

Citation 04

https://link.springer.com/article/10.1007/s42765-022-00252-6

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/

Citation 06

https://www.sciencedirect.com/science/article/pii/S0048969722056844

Citation 07

https://pubs.acs.org/doi/abs/10.1021/acsami.2c16418

Citation 08

https://www.sciencedirect.com/science/article/pii/S0049384822004807

Citation 09

https://link.springer.com/article/10.1186/s12951-022-01288-x

Citation 10

https://www.sciencedirect.com/science/article/pii/S0269749122014312

Citation 11

https://link.springer.com/article/10.1007/s00018-021-03902-x

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351451/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma and other biological fluids.

Available Sizes

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