Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Product Specifications

Abbreviation

NT-proBNP

UniProt

P16860

Cross Reactivity

This kit recognizes Human NT-proBNP in samples.No significant cross-reactivity or interference between Human NT-proBNP and analogues was observed

Target

NT-Pro-BNP

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NT-proBNP. You can calculate the concentration of Human NT-proBNP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.09 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2073-4409/11/18/2794

Citation 02

https://www.mdpi.com/2043878

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332221003164

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332221003164

Citation 05

https://www.mdpi.com/2077-0383/8/2/165

Citation 06

https://www.frontiersin.org/articles/10.3389/fphys.2022.922775/full?utm_source=dlvr.it&utm_medium=twitter

Citation 07

https://www.mdpi.com/2115728

Citation 08

https://www.mdpi.com/2227-9059/10/7/1751

Citation 09

https://www.mdpi.com/1446118

Citation 10

https://www.mdpi.com/1966912

Citation 11

https://bpspubs.onlinelibrary.wiley.com/doi/abs/10.1111/bcp.14695

Citation 12

https://www.mdpi.com/2075-4418/12/6/1435

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human NT-proBNP concentrations in serum, plasma and other biological fluids.

Available Sizes

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