Human ADP/Acrp30 (Adiponectin) ELISA Kit

Product Specifications

Abbreviation

ADP; Acrp30

UniProt

Q15848

Cross Reactivity

This kit recognizes Human ADP/Acrp30 in samples.No significant cross-reactivity or interference between Human ADP/Acrp30 and analogues was observed

Target

GBP28; ACDC; APM1; ADPN; AdipoQ; ADIPQTL1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADP/Acrp30. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADP/Acrp30. You can calculate the concentration of Human ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S001393511930862X

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349898/

Citation 03

https://www.spandidos-publications.com/10.3892/ijo.2018.4651

Citation 04

https://www.mdpi.com/706800

Citation 05

https://www.mdpi.com/1660-4601/19/22/15195

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8200454/

Citation 07

https://www.mdpi.com/1004704

Citation 08

https://www.nature.com/articles/s41598-021-87583-3

Citation 09

https://www.nature.com/articles/s41598-021-87583-3

Citation 10

https://www.sciencedirect.com/science/article/pii/S1530891X20484101

Citation 11

https://link.springer.com/article/10.1007/s40620-021-00972-9

Citation 12

https://link.springer.com/article/10.1007/s12020-022-03198-2

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human ADP/Acrp30 concentrations in serum, plasma and other biological fluids.

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