Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit

Product Specifications

Abbreviation

ICAM-1; CD54

UniProt

P05362

Cross Reactivity

This kit recognizes Human ICAM-1/CD54 in samples.No significant cross-reactivity or interference between Human ICAM-1/CD54 and analogues was observed

Target

CD54

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ICAM-1/CD54. You can calculate the concentration of Human ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2072-6643/13/11/4047

Citation 02

https://www.sciencedirect.com/science/article/pii/S0143720822003825

Citation 03

https://www.hindawi.com/journals/omcl/2019/6439021/

Citation 04

https://pubs.acs.org/doi/abs/10.1021/acs.jproteome.0c00883

Citation 05

https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/brazil-and-cashew-nuts-intake-improve-body-composition-and-endothelial-health-in-women-at-cardiometabolic-risk-brazilian-nuts-study-a-randomized-controlled-trial/C795AF78D7FC7B321A07393D0BCE1B7B

Citation 06

https://www.spandidos-publications.com/10.3892/ol.2022.13443

Citation 07

https://www.spandidos-publications.com/10.3892/etm.2023.11787

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcla.24367

Citation 09

https://www.liebertpub.com/doi/abs/10.1089/cell.2021.0043

Citation 10

https://www.sciencedirect.com/science/article/pii/S2211364920301020

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human ICAM-1/CD54 concentrations in serum, plasma and other biological fluids.

Available Sizes

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

LRRC25 (C-term) Rabbit Polyclonal Antibody
AP52528PU-N 400 µL

LRRC25 (C-term) Rabbit Polyclonal Antibody

Sign In for Pricing
View Details
RSKR Human Gene Knockout Kit (CRISPR)
KN421761 1 Kit

RSKR Human Gene Knockout Kit (CRISPR)

Sign In for Pricing
View Details
NABP1 (NM_001031716) Human Over-expression Lysate
LY422167 100 µg

NABP1 (NM_001031716) Human Over-expression Lysate

Sign In for Pricing
View Details
Thyroglobulin (2H11 + 6E1), CF640R conjugate, 0.1mg/mL
BNC400724-500 1x 500 µL

Thyroglobulin (2H11 + 6E1), CF640R conjugate, 0.1mg/mL

Sign In for Pricing
View Details
NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44]
HA722282 100 µL

NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44]

Sign In for Pricing
View Details
Elab Fluor® 488 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]
E-AB-F1132UL-01 25 µg

Elab Fluor® 488 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]

Sign In for Pricing
View Details