Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit

Product Specifications

Abbreviation

ICAM-1; CD54

UniProt

P05362

Cross Reactivity

This kit recognizes Human ICAM-1/CD54 in samples.No significant cross-reactivity or interference between Human ICAM-1/CD54 and analogues was observed

Target

CD54

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ICAM-1/CD54. You can calculate the concentration of Human ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2072-6643/13/11/4047

Citation 02

https://www.sciencedirect.com/science/article/pii/S0143720822003825

Citation 03

https://www.hindawi.com/journals/omcl/2019/6439021/

Citation 04

https://pubs.acs.org/doi/abs/10.1021/acs.jproteome.0c00883

Citation 05

https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/brazil-and-cashew-nuts-intake-improve-body-composition-and-endothelial-health-in-women-at-cardiometabolic-risk-brazilian-nuts-study-a-randomized-controlled-trial/C795AF78D7FC7B321A07393D0BCE1B7B