Human sST2 (Soluble ST2) ELISA Kit

Product Specifications

Abbreviation

SST2

UniProt

Q01638

Cross Reactivity

This kit recognizes Human sST2 in samples.No significant cross-reactivity or interference between Human sST2 and analogues was observed

Target

IL1RL1; DER4; FIT-1; IL33R; ST2L; ST2V; T1; IL-1 R4

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sST2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sST2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sST2. You can calculate the concentration of Human sST2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.jstage.jst.go.jp/article/allergolint/72/1/72_173/_article/-char/ja/

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517782/

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/ene.14419

Citation 04

https://www.jstage.jst.go.jp/article/allergolint/67/Supplement.1/67_S32/_article/-char/ja/

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1111/crj.12718

Citation 06

https://www.karger.com/Article/Abstract/488015

Citation 07

https://www.sciencedirect.com/science/article/pii/S019888592200012X

Citation 08

https://www.mdpi.com/1903182

Citation 09

https://www.scielo.br/j/rbccv/a/f9N8dnwkJ9QzhL3xTM5cmqn/?lang=en&format=html

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004757/

Citation 11

https://www.karger.com/Article/Abstract/524765

Citation 12

https://www.tandfonline.com/doi/abs/10.1080/08941939.2016.1240270

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human sST2 concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

RC230173L3 10 µg

ZMYM3 (NM_001171163) Human Tagged Lenti ORF Clone

Ask

View Details

CRJ1103-01 15 nmol

DNAH7 siRNA (Human)

Ask

View Details

CRJ1103-02 30 nmol

DNAH7 siRNA (Human)

Ask

View Details

NBP2-81086AF750 0.1 mL

Rabbit Monoclonal Integrin beta 7 Antibody (FIB504) [Alexa Fluor 750]

Ask

View Details

MBS1467261-01 0.02 mg (E-Coli)

Recombinant Alkalilimnicola ehrlichei Type III pantothenate kinase (coaX)

Ask

View Details

MBS1467261-02 0.1 mg (E-Coli)

Recombinant Alkalilimnicola ehrlichei Type III pantothenate kinase (coaX)

Ask

View Details