Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit

Product Specifications

Abbreviation

MMP-9

UniProt

P14780

Cross Reactivity

This kit recognizes Human MMP-9 in samples.No significant cross-reactivity or interference between Human MMP-9 and analogues was observed

Target

MMP9; CLG4B; Gelatinase B; GELB; MANDP2;92kDa Type IV Collagenase;92 KDa Gelatinase

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-9. You can calculate the concentration of Human MMP-9 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0168827819302995

Citation 02

https://www.nature.com/articles/s41467-021-23789-3

Citation 03

https://stke.sciencemag.org/content/9/448/ra98.short

Citation 04

https://www.mdpi.com/1400396

Citation 05

https://www.mdpi.com/2161798

Citation 06

https://pubs.rsc.org/en/content/articlelanding/2022/tb/d2tb00519k

Citation 07

https://www.spandidos-publications.com/10.3892/ijo.2014.2645?text=abstract

Citation 08

https://www.sciencedirect.com/science/article/pii/S0891584915005158

Citation 09

https://www.sciencedirect.com/science/article/pii/S0009279721003756

Citation 10

https://www.frontiersin.org/articles/10.3389/fimmu.2020.01902/full

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8506992/

Citation 12

https://europepmc.org/article/med/34340266?javascript_support=no

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human MMP-9 concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

HY-P72613-01 2 µg

IFN-alpha/beta R2, Human (HEK293, His)

Ask

View Details

HY-P72613-02 10 µg

IFN-alpha/beta R2, Human (HEK293, His)

Ask

View Details

HY-P72613-03 50 µg

IFN-alpha/beta R2, Human (HEK293, His)

Ask

View Details

HY-P72613-04 100 µg

IFN-alpha/beta R2, Human (HEK293, His)

Ask

View Details

MBS2709408-01 24 Strip Well

Mini Samples Gastric Inhibitory Polypeptide (GIP) ELISA Kit

Ask

View Details

MBS2709408-02 48 Well

Mini Samples Gastric Inhibitory Polypeptide (GIP) ELISA Kit

Ask

View Details