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Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Product Specifications

Abbreviation

HIF-1α

UniProt

Q16665

Cross Reactivity

This kit recognizes Human HIF-1α in samples.No significant cross-reactivity or interference between Human HIF-1α and analogues was observed

Target

HIF1A; HIF-1A; MOP1; PASD8; bHLHe78; basic helix-loop-helix transcription factor

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HIF-1α. You can calculate the concentration of Human HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Product Datasheet

https://789.bio/ea/hmh84s

Product MSDS

https://789.bio/eb/qPmjXL

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1111/jre.12973

Citation 02

https://www.nature.com/articles/s41598-021-01695-4

Citation 03

https://link.springer.com/article/10.1007/s10803-020-04820-w

Citation 04

https://www.nature.com/articles/s41598-022-16053-1

Citation 05

https://www.sciencedirect.com/science/article/pii/S1818087619308839

Citation 06

https://www.sciencedirect.com/science/article/pii/S2405844020311300

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8848409/

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.25885

Citation 09

https://www.sciencedirect.com/science/article/pii/S0024320517303028

Citation 10

https://www.spandidos-publications.com/10.3892/ijmm.2018.3664

Citation 11

https://link.springer.com/article/10.1186/s12883-021-02369-4

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcla.24073

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human HIF-1α concentrations in serum, plasma and other biological fluids.

Available Sizes

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