Human MMP-13 (Matrix Metalloproteinase 13) ELISA Kit

Product Specifications

Abbreviation

MMP-13

UniProt

P45452

Cross Reactivity

This kit recognizes Human MMP-13 in samples.No significant cross-reactivity or interference between Human MMP-13 and analogues was observed

Target

MMP13; CLG3; MANDP1; Collagenase 3

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-13. You can calculate the concentration of Human MMP-13 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1063458418314602

Citation 02

https://www.frontiersin.org/articles/10.3389/fphar.2021.656124/full

Citation 03

https://www.sciencedirect.com/science/article/pii/S1063458417310270

Citation 04

https://www.sciencedirect.com/science/article/pii/S1567576919303856

Citation 05

https://www.hindawi.com/journals/ppar/2019/6403504/abs/

Citation 06

https://www.sciencedirect.com/science/article/pii/S1529943022000353

Citation 07

https://www.sciencedirect.com/science/article/pii/S0753332218323953

Citation 08

https://www.sciencedirect.com/science/article/pii/S0014482717304238

Citation 09

https://www.sciencedirect.com/science/article/pii/S0944711318305725

Citation 10

https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.1997090

Citation 11

https://journals.sagepub.com/doi/abs/10.1177/19476035221142011

Citation 12

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238449

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human MMP-13 concentrations in serum, plasma and other biological fluids.

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