Human OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit

Product Specifications

Abbreviation

OxLDL

Cross Reactivity

This kit recognizes Human OxLDL in samples.No significant cross-reactivity or interference between Human OxLDL and analogues was observed

Target

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OxLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OxLDL. You can calculate the concentration of Human OxLDL in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubmed.ncbi.nlm.nih.gov/36420478/

Citation 02

https://www.liebertpub.com/doi/abs/10.1089/ars.2022.0065

Citation 03

https://www.nature.com/articles/s41388-019-1015-z

Citation 04

https://www.sciencedirect.com/science/article/pii/S003991402200995X

Citation 05

https://www.frontiersin.org/articles/10.3389/fnut.2021.668622/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Nutrition&id=668622