Human MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

Product Specifications

Abbreviation

MCP-1

UniProt

P13500

Cross Reactivity

This kit recognizes Human MCP-1 in samples.No significant cross-reactivity or interference between Human MCP-1 and analogues was observed

Target

CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MCP-1. You can calculate the concentration of Human MCP-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-021-01369-9

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/

Citation 03

https://www.sciencedirect.com/science/article/pii/S0049384822004807

Citation 04

https://www.nature.com/articles/s41419-020-2665-z

Citation 05

https://clincancerres.aacrjournals.org/content/25/6/1957.abstract

Citation 06

https://europepmc.org/article/pmc/pmc9519340?javascript_support=no

Citation 07

https://www.mdpi.com/2098480

Citation 08

https://www.mdpi.com/1871286

Citation 09

https://www.mdpi.com/2013838

Citation 10

https://www.sciencedirect.com/science/article/pii/S0378874122004305

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576104/

Citation 12

https://link.springer.com/article/10.1007/s00011-021-01459-w

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human MCP-1 concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MBS8527817-01 0.1 mL

Mouse Monoclonal Antibody to ONECUT3

Ask

View Details

MBS8527817-02 0.1 mL (AF405L)

Mouse Monoclonal Antibody to ONECUT3

Ask

View Details

MBS8527817-03 0.1 mL (AF405S)

Mouse Monoclonal Antibody to ONECUT3

Ask

View Details

MBS8527817-04 0.1 mL (AF610)

Mouse Monoclonal Antibody to ONECUT3

Ask

View Details

MBS8527817-05 0.1 mL (AF635)

Mouse Monoclonal Antibody to ONECUT3

Ask

View Details

MBS8527817-06 0.1 mL (APC)

Mouse Monoclonal Antibody to ONECUT3

Ask

View Details