Human Zonulin ELISA Kit

Product Specifications

Abbreviation

Zonulin

UniProt

P00738

Cross Reactivity

This kit recognizes Human Zonulin in samples.No significant cross-reactivity or interference between Human Zonulin and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Zonulin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Zonulin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Zonulin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Zonulin. You can calculate the concentration of Human Zonulin in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.47 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.31056

Citation 02

https://www.frontiersin.org/articles/10.3389/fimmu.2021.579140/full

Citation 03

https://www.sciencedirect.com/science/article/pii/S0308814621006890

Citation 04

https://www.liebertpub.com/doi/abs/10.1089/ars.2020.8109

Citation 05

https://www.mdpi.com/article/10.3390/nu15010145

Citation 06

https://www.mdpi.com/1802306

Citation 07

https://www.mdpi.com/1015814

Citation 08

https://www.sciencedirect.com/science/article/pii/S1043661819326568

Citation 09

https://www.liebertpub.com/doi/abs/10.1089/ars.2018.7596

Citation 10

https://www.mdpi.com/973710

Citation 11

https://www.mdpi.com/2072-6643/14/10/2153

Citation 12

https://www.hindawi.com/journals/omcl/2020/8630275/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human Zonulin concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

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