Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit

Product Specifications

Abbreviation

SRANKL

UniProt

O14788

Cross Reactivity

This kit recognizes Human sRANKL in samples. No significant cross-reactivity or interference between Human sRANKL and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sRANKL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sRANKL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sRANKL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sRANKL. You can calculate the concentration of Human sRANKL in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8335545/

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11990

Citation 03

https://www.frontiersin.org/articles/10.3389/fphar.2020.609119/full

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118287/

Citation 05

https://europepmc.org/article/pmc/pmc9908480?javascript_support=no

Citation 06

https://www.hindawi.com/journals/jir/2019/6195258/abs/

Citation 07

https://www.sciencedirect.com/science/article/pii/S1043466618304095

Citation 08

https://www.sciencedirect.com/science/article/pii/S0099239919306995

Citation 09

https://journals.sagepub.com/doi/abs/10.1177/1534734619859730

Citation 10

https://journals.lww.com/jpho-online/Fulltext/9900/OPG_RANK_RANKL_Axis_in_Egyptian_Children_With.151.aspx

Citation 11

https://bmcmusculoskeletdisord.biomedcentral.com/articles/10.1186/s12891-018-2356-4

Citation 12

https://www.jstage.jst.go.jp/article/josnusd/59/3/59_16-0677/_article/-char/ja/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human sRANKL concentrations in serum, plasma and other biological fluids.

Available Sizes

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