Human Ang1-7 (Angiotensin 1-7) ELISA Kit

Product Specifications

Abbreviation

Ang1-7

UniProt

P01019

Cross Reactivity

This kit recognizes Human Ang1-7 in samples.No significant cross-reactivity or interference between Human Ang1-7 and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang1-7. During the reaction, Human Ang1-7 in the sample or standard competes with a fixed amount of Human Ang1-7 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang1-7. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ang1-7 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://cardiab.biomedcentral.com/articles/10.1186/s12933-022-01573-x

Citation 02

https://link.springer.com/article/10.1186/s12575-022-00177-9

Citation 03

https://static.frontiersin.org/articles/10.3389/fendo.2022.912064/full

Citation 04

https://www.mdpi.com/1422-0067/21/11/3861

Citation 05

https://www.sciencedirect.com/science/article/pii/S0939475322000461

Citation 06

https://www.sciencedirect.com/science/article/pii/S0753332217342154

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human Ang1-7 concentrations in serum, plasma and other biological fluids.

Available Sizes

More Discoveries

Explore Other Products

Browse additional items from our catalog

41197011 1.0 µg DNA

RPL23AP7 ORF Vector (Human) (pORF)

View Details

abx900748-01 15 nmol

Human CALCRL siRNA

View Details

abx900748-02 30 nmol

Human CALCRL siRNA

View Details

FITC-30294-01 20 Tests

FITC Mouse IgG3, κ Isotype Control (A112-3)

View Details

FITC-30294-02 100 Tests

FITC Mouse IgG3, κ Isotype Control (A112-3)

View Details

TU152578 1.0 mL

BC021891 3'UTR GFP Stable Cell Line

View Details