Human KL (Klotho) ELISA Kit

Product Specifications

Abbreviation

UniProt

Q9UEF7

Cross Reactivity

This kit recognizes Human KL in samples. No significant cross-reactivity or interference between Human KL and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KL. You can calculate the concentration of Human KL in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://ard.bmj.com/content/81/Suppl_1/1248.1.abstract

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/

Citation 03

https://pubmed.ncbi.nlm.nih.gov/35349421/

Citation 04

https://www.frontiersin.org/articles/10.3389/fphys.2022.856088/full?utm_source=dlvr.it&utm_medium=twitter

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8573245/

Citation 06

https://www.karger.com/Article/Abstract/492244

Citation 07

https://link.springer.com/article/10.1007/s12032-021-01595-7

Citation 08

https://link.springer.com/article/10.1007/s40620-021-00972-9

Citation 09

https://link.springer.com/article/10.1007/s00005-023-00669-w

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526019/

Citation 11

https://peerj.com/articles/12295/

Citation 12

https://link.springer.com/article/10.1186/s12882-022-02773-z

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human KL concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

NBP2-49371PEP 0.1 mL

GIPC2 Recombinant Protein Antigen

Ask

View Details

RC206731L3V 200 µL

CCDC116 (NM_152612) Human Tagged ORF Clone Lentiviral Particle

Ask

View Details

CAC15515-01 50 µg

GUCY2D Polyclonal Antibody

Ask

View Details

CAC15515-02 100 µg

GUCY2D Polyclonal Antibody

Ask

View Details

454550 10 mg

Methy 2-Methoxyphenyl Adapalene

Ask

View Details

RC200070L2 10 µg

AATF (NM_012138) Human Tagged Lenti ORF Clone

Ask

View Details