Human hs-CRP (high-sensitivity C-Reactive Protein) ELISA Kit

Product Specifications

Abbreviation

Hs-CRP

UniProt

P02741

Cross Reactivity

This kit recognizes Human hs-CRP in samples. No significant cross-reactivity or interference between Human hs-CRP and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human hs-CRP. You can calculate the concentration of Human hs-CRP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://academic.oup.com/jid/advance-article-abstract/doi/10.1093/infdis/jiac287/6633821

Citation 02

https://europepmc.org/article/pmc/pmc9521405?javascript_support=no

Citation 03

https://link.springer.com/article/10.1186/s40798-019-0212-1

Citation 04

https://www.mdpi.com/1133804

Citation 05

https://www.sciencedirect.com/science/article/pii/S1756464622004625

Citation 06

https://www.mdpi.com/650654

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8200454/

Citation 08

https://www.mdpi.com/2072-6643/10/6/724/htm

Citation 09

https://www.mdpi.com/1446118

Citation 10

https://www.hindawi.com/journals/mi/2019/4756295/abs/

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1111/hiv.12884

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1111/dth.14733

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human hs-CRP concentrations in serum, plasma and other biological fluids.

Available Sizes

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