Human INS (Insulin) ELISA Kit

Product Specifications

Abbreviation

INS

UniProt

P01308

Cross Reactivity

This kit recognizes Human INS in samples. No significant cross-reactivity or interference between Human INS and analogues was observed

Target

IDDM2; ILPR; IRDN; MODY10

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human INS. You can calculate the concentration of Human INS in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50 μIU/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.47 μIU/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.science.org/doi/abs/10.1126/sciadv.add3197

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202200481

Citation 03

https://www.sciencedirect.com/science/article/pii/S1319562X21001376

Citation 04

https://www.mdpi.com/2072-6643/12/9/2644

Citation 05

https://www.mdpi.com/2072-6643/12/12/3659

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8200454/

Citation 07

https://link.springer.com/article/10.1007/s00726-020-02891-8

Citation 08

https://www.sciencedirect.com/science/article/pii/S0377840121001395

Citation 09

https://www.sciencedirect.com/science/article/pii/S0168822719302177

Citation 10

https://www.hindawi.com/journals/mi/2019/4756295/abs/

Citation 11

https://www.tandfonline.com/doi/abs/10.1080/01480545.2022.2058010

Citation 12

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0274663

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human INS concentrations in serum, plasma and other biological fluids.

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