Human IL-35 (Interleukin 35) ELISA Kit

Product Specifications

Abbreviation

IL-35

UniProt

P29459, Q14213

Cross Reactivity

This kit recognizes Human IL-35 in samples. No significant cross-reactivity or interference between Human IL-35 and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-35. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-35 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-35, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-35. You can calculate the concentration of Human IL-35 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://gut.bmj.com/content/early/2021/08/02/gutjnl-2021-324071.abstract

Citation 02

https://www.nature.com/articles/s41467-022-28338-0

Citation 03

https://link.springer.com/article/10.1186/s12974-019-1606-1

Citation 04

https://www.nature.com/articles/s41598-019-52286-3

Citation 05

https://www.mdpi.com/688776

Citation 06

https://link.springer.com/article/10.1007/s13277-015-4616-3

Citation 07

https://link.springer.com/article/10.1007/s10067-022-06137-8

Citation 08

https://www.liebertpub.com/doi/abs/10.1089/vim.2020.0176

Citation 09

https://www.spandidos-publications.com/10.3892/ol.2017.5949

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783395/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IL-35 concentrations in serum, plasma and other biological fluids.

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