Human ITLN1 (Intelectin 1/Omentin) ELISA Kit

Product Specifications

Abbreviation

ITLN1

UniProt

Q8WWA0

Cross Reactivity

This kit recognizes Human ITLN1 in samples. No significant cross-reactivity or interference between Human ITLN1 and analogues was observed

Target

HL-1; HL1; INTL; ITLN; LFR; hIntL; Intelectin 1; Galactofuranose Binding; Intestinal Lactoferrin Receptor

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ITLN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ITLN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ITLN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ITLN1. You can calculate the concentration of Human ITLN1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.63-40 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.38 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://cardiab.biomedcentral.com/articles/10.1186/s12933-020-01151-z

Citation 02

https://europepmc.org/article/med/35222038?javascript_support=no

Citation 03

https://www.sciencedirect.com/science/article/pii/S0047637422000598

Citation 04

https://cardiab.biomedcentral.com/articles/10.1186/s12933-019-0880-7

Citation 05

https://www.tandfonline.com/doi/abs/10.1080/09546634.2021.1901845

Citation 06

https://link.springer.com/article/10.1007/s00592-019-01355-0

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/dth.14378

Citation 08

https://www.tandfonline.com/doi/abs/10.1080/02770903.2017.1355378

Citation 09

https://pubmed.ncbi.nlm.nih.gov/35227049/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human ITLN1 concentrations in serum, plasma and other biological fluids.

Available Sizes

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