Human MT (Melatonin) ELISA Kit

Product Specifications

Abbreviation

Cross Reactivity

This kit recognizes Human MT in samples.No significant cross-reactivity or interference between Human MT and analogues was observed

Target

N-Acetyl-5-Methoxytryptamine

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human MT. During the reaction, Human MT in the sample or standard competes with a fixed amount of Human MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12657

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/ctm2.746

Citation 03

https://www.sciencedirect.com/science/article/pii/S1672022921000188

Citation 04

https://www.frontiersin.org/articles/10.3389/fnut.2022.973291/full

Citation 05

https://www.mdpi.com/2073-4409/11/10/1690

Citation 06

https://www.mdpi.com/2072-6643/14/1/117

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519399/

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695404/

Citation 09

https://www.sciencedirect.com/science/article/pii/S1567724921000362

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1111/aji.13585

Citation 11

https://www.mdpi.com/1095382

Citation 12

https://link.springer.com/article/10.1007/s00508-022-02109-9

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human MT concentrations in serum, plasma and other biological fluids.

Available Sizes

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