Human A-GHRL (Acylated Ghrelin) ELISA Kit
Product Specifications
Abbreviation
A-GHRL
UniProt
Q9UBU3
Cross Reactivity
This kit recognizes Human A-GHRL in samples. No significant cross-reactivity or interference between Human A-GHRL and analogues was observed
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Metabolism; Signal transduction; Neuroscience; Cardiovascular
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human A-GHRL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human A-GHRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human A-GHRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human A-GHRL. You can calculate the concentration of Human A-GHRL in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.38 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S1871403X21000910
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human A-GHRL concentrations in serum, plasma and other biological fluids.
Available Sizes
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