Human PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Product Specifications

Abbreviation

PTGS2; COX-2

UniProt

P35354

Cross Reactivity

This kit recognizes Human PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Human PTGS2/COX-2 and analogues was observed

Target

COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PTGS2/COX-2. You can calculate the concentration of Human PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/978204

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310101/

Citation 03

https://iovs.arvojournals.org/article.aspx?articleid=2783636

Citation 04

https://www.mdpi.com/1420-3049/27/13/4264

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695383/

Citation 06

https://www.frontiersin.org/articles/10.3389/fphar.2020.01155/full

Citation 07

https://www.mdpi.com/2218-273X/10/1/108

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcpt.13471

Citation 09

https://link.springer.com/article/10.1186/s12906-020-03092-2

Citation 10

https://www.sciencedirect.com/science/article/pii/S1098882316300855

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human PTGS2/COX-2 concentrations in serum, plasma and other biological fluids.

Available Sizes

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