Human VF (Visfatin) ELISA Kit

Product Specifications

Abbreviation

UniProt

P43490

Cross Reactivity

This kit recognizes Human VF in samples. No significant cross-reactivity or interference between Human VF and analogues was observed

Target

Nampt; NAmPRTase; PBEF1; pre-B-cell colony-enhancing factor 1; Nicotinamide Phosphoribosyltransferase

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Immunology; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VF. You can calculate the concentration of Human VF in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0022395622006008

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349898/

Citation 03

https://www.nature.com/articles/s41598-021-87583-3

Citation 04

https://www.mdpi.com/1347594

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7415233/

Citation 06

https://www.sciencedirect.com/science/article/pii/S0168822719302050

Citation 07

https://www.sciencedirect.com/science/article/pii/S105687272100060X

Citation 08

https://www.sciencedirect.com/science/article/pii/S1871403X2200045X

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1111/dth.14378

Citation 10

https://link.springer.com/article/10.1007/s00403-018-1867-z

Citation 11

https://link.springer.com/article/10.1186/s12944-019-1169-2

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359883/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human VF concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

NBP2-90322JF549 0.1 mL

Rabbit Monoclonal CDNF Antibody (104) [Janelia Fluor 549]

Ask

View Details

PM44078M5 20 µg

Rffl (NM_026097) Mouse Recombinant Protein

Ask

View Details

MBS7159983 Inquire

Rabbit anti-Thermus thermophilus (strain HB8/27634/DSM 579) polA Polyclonal Antibody

Ask

View Details

AM09252PU-N 500 µg

Lung Carcinoma Mouse Monoclonal Antibody [Clone ID: CHALU2]

Ask

View Details

TA305694 100 µg

Rapsyn (RAPSN) Goat Polyclonal Antibody

Ask

View Details