Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit

Product Specifications

Abbreviation

ESM1

UniProt

Q9NQ30

Cross Reactivity

This kit recognizes Human ESM1 in samples. No significant cross-reactivity or interference between Human ESM1 and analogues was observed

Target

Endocan; Endothelial cell-specific molecule 1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Tags and Cell Markers

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ESM1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ESM1. You can calculate the concentration of Human ESM1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://aap.onlinelibrary.wiley.com/doi/abs/10.1902/jop.2016.160279

Citation 02

https://journals.sagepub.com/doi/abs/10.1177/00033197211012135

Citation 03

https://journals.sagepub.com/doi/abs/10.1177/0003319721995664

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197208/

Citation 05

https://www.spandidos-publications.com/10.3892/etm.2021.9646

Citation 06

https://journals.sagepub.com/doi/abs/10.1177/1933719118773480

Citation 07

https://www.tandfonline.com/doi/abs/10.1080/14767058.2019.1649388

Citation 08

https://www.tandfonline.com/doi/abs/10.1080/14767058.2018.1548597

Citation 09

https://www.degruyter.com/view/journals/jpme/47/5/article-p510.xml

Citation 10

https://journals.lww.com/md-journal/Fulltext/2022/12300/The_investigation_of_levels_of_endothelial.28.aspx

Citation 11

https://www.tandfonline.com/doi/abs/10.1080/01443615.2020.1819215

Citation 12

https://www.tandfonline.com/doi/abs/10.1080/01443615.2020.1789952

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human ESM1 concentrations in serum, plasma and other biological fluids.

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