Human NFKB-p65 (Nuclear factor NF-kappa-B p65 subunit) ELISA Kit

Product Specifications

Abbreviation

NFKB-p65

UniProt

Q04206

Cross Reactivity

This kit recognizes Human NFKB-p65 in samples. No significant cross-reactivity or interference between Human NFKB-p65 and analogues was observed

Target

NFKB3; p65; RelA

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Microbiology; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NFKB-p65. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NFKB-p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NFKB-p65. You can calculate the concentration of Human NFKB-p65 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.frontiersin.org/articles/10.3389/fphar.2021.722126/full

Citation 02

https://www.sciencedirect.com/science/article/pii/S0009279721001745

Citation 03

https://www.mdpi.com/2072288

Citation 04

https://www.futuremedicine.com/doi/abs/10.2217/epi-2022-0430

Citation 05

https://www.sciencedirect.com/science/article/pii/S0165037820301133

Citation 06

https://www.mdpi.com/2075-1729/12/10/1462

Citation 07

https://www.sciencedirect.com/science/article/pii/S0887233321000680

Citation 08

https://www.liebertpub.com/doi/abs/10.1089/jir.2022.0110

Citation 09

https://portlandpress.com/bioscirep/article-abstract/39/11/BSR20191252/220754

Citation 10

https://www.spandidos-publications.com/mmr/14/1/307?text=fulltext

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human NFKB-p65 concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

CQA5919-01 30 µL

Anti-SLC23A2 Antibody

Ask

View Details

CQA5919-02 50 μL

Anti-SLC23A2 Antibody

Ask

View Details

CQA5919-03 100 µL

Anti-SLC23A2 Antibody

Ask

View Details

CQA5919-04 200 µL

Anti-SLC23A2 Antibody

Ask

View Details

GA218171 1 Kit

Mouse Ighv1-15 activation kit by CRISPRa

Ask

View Details

RC203981L3 10 µg

GNL3L (NM_019067) Human Tagged Lenti ORF Clone

Ask

View Details