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Human ON (Osteonectin) ELISA Kit

Product Specifications

Abbreviation

ON

UniProt

P09486

Cross Reactivity

This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed

Target

SPARC

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ON. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ON and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ON, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ON. You can calculate the concentration of Human ON in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Product Datasheet

https://789.bio/ea/apcw98

Product MSDS

https://789.bio/eb/jb9ubH

Citation 01

https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep4.1234

Citation 02

https://www.sciencedirect.com/science/article/pii/S0009279721003124

Citation 03

https://www.sciencedirect.com/science/article/pii/S0928098721000701

Citation 04

https://www.sciencedirect.com/science/article/pii/S1773224722003252

Citation 05

https://www.sciencedirect.com/science/article/pii/S0196978123000360

Citation 06

https://www.sciencedirect.com/science/article/pii/S0024320520312558

Citation 07

https://www.sciencedirect.com/science/article/pii/S0024320520307438

Citation 08

https://www.sciencedirect.com/science/article/pii/S1359511319304180

Citation 09

https://www.sciencedirect.com/science/article/pii/S187881812030815X

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human ON concentrations in serum, plasma and other biological fluids.

Available Sizes

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