Human OC/BGP (Osteocalcin) ELISA Kit

Product Specifications

Abbreviation

OC; BGP

UniProt

P02818

Cross Reactivity

This kit recognizes Human OC/BGP in samples. No significant cross-reactivity or interference between Human OC/BGP and analogues was observed

Target

BGLAP; OT; Bone Gamma-carboxyglutamate Protein; Bone Gla Protein

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OC/BGP. You can calculate the concentration of Human OC/BGP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.25-80 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.75 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0928493120337759

Citation 02

https://www.sciencedirect.com/science/article/pii/S0928493121004379

Citation 03

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-021-02312-x

Citation 04

https://www.sciencedirect.com/science/article/pii/S0955286322003138

Citation 05

https://www.sciencedirect.com/science/article/pii/S0009279721003124

Citation 06

https://www.sciencedirect.com/science/article/pii/S0928493117336299

Citation 07

https://www.sciencedirect.com/science/article/pii/S0928493118314607

Citation 08

https://www.sciencedirect.com/science/article/pii/S0928493119321824

Citation 09

https://www.sciencedirect.com/science/article/pii/S0141813019336372

Citation 10

https://www.sciencedirect.com/science/article/pii/S0141813018345987

Citation 11

https://www.sciencedirect.com/science/article/pii/S0141813019341716

Citation 12

https://www.sciencedirect.com/science/article/pii/S8756328219300778

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human OC/BGP concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

C1042S 20 μL

ER-Tracker Green

Ask

View Details

ELI-26096h 96 Tests

Human EID3 ELISA KIT

Ask

View Details

C2409-14R 100 Tests

CD54 (Intercellular Adhesion Molecule, ICAM-1) (PE)

Ask

View Details

425260-01 100 mg

3-Cyano-N-cyclopropylbenzamide

Ask

View Details

425260-02 250 mg

3-Cyano-N-cyclopropylbenzamide

Ask

View Details