Human OPG (Osteoprotegerin) ELISA Kit

Product Specifications

Abbreviation

OPG

UniProt

O00300

Cross Reactivity

This kit recognizes Human OPG in samples. No significant cross-reactivity or interference between Human OPG and analogues was observed

Target

TNFRSF11B; OCIF; TR1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Immunology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OPG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OPG. You can calculate the concentration of Human OPG in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663525/

Citation 02

https://europepmc.org/article/med/35222038?javascript_support=no

Citation 03

https://www.nature.com/articles/s41598-017-16788-2?elqTrackId=93f3b50d3c804e249efae9c74b569675

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118287/

Citation 05

https://www.sciencedirect.com/science/article/pii/S1043466618304095

Citation 06

https://www.hindawi.com/journals/jir/2019/6195258/abs/

Citation 07

https://www.sciencedirect.com/science/article/pii/S0099239919306995

Citation 08

https://www.tandfonline.com/doi/abs/10.1080/00365513.2020.1728786

Citation 09

https://journals.lww.com/jpho-online/Fulltext/9900/OPG_RANK_RANKL_Axis_in_Egyptian_Children_With.151.aspx

Citation 10

https://europepmc.org/article/med/34722902?javascript_support=no

Citation 11

https://academic.oup.com/jes/article-abstract/3/8/1617/5525626

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human OPG concentrations in serum, plasma and other biological fluids.

Available Sizes

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