Human S100B (S100 Calcium Binding Protein B) ELISA Kit

Product Specifications

Abbreviation

S100B

UniProt

P04271

Cross Reactivity

This kit recognizes Human S100B in samples. No significant cross-reactivity or interference between Human S100B and analogues was observed

Target

S100-B; NEF; S100Beta

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human S100B. You can calculate the concentration of Human S100B in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2072-6643/13/11/4047

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381989/

Citation 03

https://www.liebertpub.com/doi/full/10.1089/jir.2022.0098

Citation 04

https://www.hindawi.com/journals/mi/2017/3605350/abs/

Citation 05

https://www.sciencedirect.com/science/article/pii/S0022510X22001861

Citation 06

https://link.springer.com/article/10.1186/s12883-022-02686-2

Citation 07

https://link.springer.com/article/10.1007/s10096-019-03569-0

Citation 08

https://link.springer.com/content/pdf/10.1007/s10072-020-04323-7.pdf

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1111/jir.12896

Citation 10

https://www.tandfonline.com/doi/abs/10.1080/02699052.2021.1931449

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238324/

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9012913/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human S100B concentrations in serum, plasma and other biological fluids.

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