Human SEMA3A (Semaphorin 3A) ELISA Kit

Product Specifications

Abbreviation

SEMA3A

UniProt

Q14563

Cross Reactivity

This kit recognizes Human SEMA3A in samples. No significant cross-reactivity or interference between Human SEMA3A and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA3A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA3A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SEMA3A. You can calculate the concentration of Human SEMA3A in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41413-020-0096-1

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868739/

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/jre.12995

Citation 04

https://link.springer.com/article/10.1007/s00296-022-05197-y

Citation 05

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250663

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.b.33730

Citation 07

https://www.hindawi.com/journals/ije/2022/2504660/

Citation 08

https://www.sciencedirect.com/science/article/pii/S0378111917300872

Citation 09

https://link.springer.com/article/10.1007/s10792-022-02599-6

Citation 10

https://anatomypubs.onlinelibrary.wiley.com/doi/abs/10.1002/ar.24016

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6661522/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human SEMA3A concentrations in serum, plasma and other biological fluids.

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