Human RETN (Resistin) ELISA Kit

Product Specifications

Abbreviation

RETN

UniProt

Q9HD89

Cross Reactivity

This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed

Target

ADSF; FIZZ3; RETN1; RSTN; XCP1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RETN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RETN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RETN. You can calculate the concentration of Human RETN in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389046/

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7844394/

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.16275

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349898/

Citation 05

https://www.nature.com/articles/s41598-021-87583-3

Citation 06

https://link.springer.com/article/10.1007/s40620-021-00972-9

Citation 07

https://www.sciencedirect.com/science/article/pii/S0168822719302050

Citation 08

https://www.tandfonline.com/doi/abs/10.1080/00365521.2020.1737727

Citation 09

https://www.sciencedirect.com/science/article/pii/S1871403X2200045X

Citation 10

https://europepmc.org/article/med/32991315?javascript_support=no

Citation 11

https://link.springer.com/article/10.1186/s12944-019-1169-2

Citation 12

https://journals.viamedica.pl/endokrynologia_polska/article/view/72825

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human RETN concentrations in serum, plasma and other biological fluids.

Available Sizes

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