Human FGF23 (Fibroblast Growth Factor 23) ELISA Kit

Product Specifications

Abbreviation

FGF23

UniProt

Q9GZV9

Cross Reactivity

This kit recognizes Human FGF23 in samples. No significant cross-reactivity or interference between Human FGF23 and analogues was observed

Target

FGF-23; ADHR; FGFN; HYPF; HPDR2; PHPTC

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGF23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGF23. You can calculate the concentration of Human FGF23 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://elifesciences.org/articles/52709

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8573245/

Citation 03

https://www.karger.com/Article/Abstract/492244

Citation 04

https://link.springer.com/article/10.1007/s40620-021-00972-9

Citation 05

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0280378

Citation 06

https://peerj.com/articles/12295/

Citation 07

https://bmjpaedsopen.bmj.com/content/7/1/e001753.abstract

Citation 08

https://link.springer.com/article/10.1007/s00774-021-01248-9

Citation 09

https://www.sciencedirect.com/science/article/pii/S1094695020300913

Citation 10

https://www.hindawi.com/journals/cmmm/2021/2464821/

Citation 11

https://link.springer.com/article/10.1007/s00296-019-04298-5

Citation 12

https://www.karger.com/Article/Abstract/508061

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human FGF23 concentrations in serum, plasma and other biological fluids.

Available Sizes

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