Human CPP (Copeptin) ELISA Kit

Product Specifications

Abbreviation

CPP

UniProt

P01185

Cross Reactivity

This kit recognizes Human CPP in samples.No significant cross-reactivity or interference between Human CPP and analogues was observed

Target

CT-proAVP; C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human CPP. During the reaction, Human CPP in the sample or standard competes with a fixed amount of Human CPP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human CPP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human CPP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://journals.lww.com/jhypertension/Fulltext/9900/The_relationship_between_arginine_vasopressin_gene.189.aspx

Citation 02

https://journals.lww.com/jhypertension/Abstract/2017/09002/_OP_1A_03__COPEPTIN_LEVELS_IN_PATIENTS_WITH.3.aspx

Citation 03

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250035

Citation 04

https://www.mdpi.com/2075-4418/12/6/1435

Citation 05

https://www.mdpi.com/1251082

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1111/jch.13374

Citation 07

https://journals.viamedica.pl/cardiology_journal/article/view/41893

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731524/

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1111/ped.15355

Citation 10

https://www.sciencedirect.com/science/article/pii/S0735675720303478

Citation 11

https://www.degruyter.com/document/doi/10.1515/tjb-2020-45s208/html

Citation 12

https://clinicalhypertension.biomedcentral.com/articles/10.1186/s40885-022-00221-6

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human CPP concentrations in serum, plasma and other biological fluids.

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