Human CTXI (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit

Product Specifications

Abbreviation

CTXI

UniProt

P02452 (COL1A1)

Cross Reactivity

This kit recognizes Human CTXI in samples. No significant cross-reactivity or interference between Human CTXI and analogues was observed

Target

CTX-I; CTX1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Metabolism; Cardiovascular; Cancer

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTXI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTXI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTXI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTXI. You can calculate the concentration of Human CTXI in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0955286322003138

Citation 02

https://www.sciencedirect.com/science/article/pii/S1063458417310270

Citation 03

https://www.mdpi.com/2218-273X/10/3/380

Citation 04

https://link.springer.com/article/10.1007/s13577-022-00825-y

Citation 05

https://link.springer.com/article/10.1007/s00223-019-00524-1

Citation 06

https://www.mdpi.com/1327874

Citation 07

https://www.hindawi.com/journals/bmri/2020/7917309/

Citation 08

https://link.springer.com/article/10.1007/s10067-019-04718-8

Citation 09

https://link.springer.com/article/10.1007/s11657-021-01022-4

Citation 10

https://www.hindawi.com/journals/bmri/2018/4864952/abs/

Citation 11

https://meridian.allenpress.com/angle-orthodontist/article-abstract/89/4/630/9963

Citation 12

https://www.sciencedirect.com/science/article/pii/S1991790218311073

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human CTXI concentrations in serum, plasma and other biological fluids.

Available Sizes

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