Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit

Product Specifications

Abbreviation

NOS2; iNOS

UniProt

P35228

Cross Reactivity

This kit recognizes Human NOS2/iNOS in samples. No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed

Target

NOS2A; HEP-NOS; I-NOS

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NOS2/iNOS. You can calculate the concentration of Human NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://academic.oup.com/jid/article-abstract/216/7/877/4085083

Citation 02

https://www.mdpi.com/978204

Citation 03

https://www.frontiersin.org/articles/10.3389/fimmu.2018.01500/full

Citation 04

https://link.springer.com/article/10.1007/s11356-022-24590-5

Citation 05

http://www.jneuropsychiatry.org/peer-review/plasma-pregnancyassociated-plasma-protein-a-and-cd40-are-potential-biomarkers-of-vulnerable-plaque-associated-with-middle-cerebral-12200.html