Human Ang-II (Angiotensin II) ELISA Kit

Product Specifications

Abbreviation

Ang-II

UniProt

P01019

Cross Reactivity

This kit recognizes Human Ang-II in samples.No significant cross-reactivity or interference between Human Ang-II and analogues was observed

Target

Angiotensin-2

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang-II. During the reaction, Human Ang-II in the sample or standard competes with a fixed amount of Human Ang-II on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang-II. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ang-II in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1186/s10194-021-01306-7

Citation 02

https://www.mdpi.com/article/10.3390/ijms24010343

Citation 03

https://static.frontiersin.org/articles/10.3389/fendo.2022.912064/full

Citation 04

https://europepmc.org/article/pmc/pmc8866968?javascript_support=no

Citation 05

https://link.springer.com/article/10.1186/s12967-021-02755-z

Citation 06

https://www.mdpi.com/1422-0067/21/11/3861

Citation 07

https://www.sciencedirect.com/science/article/pii/S0939475322000461

Citation 08

https://www.hindawi.com/journals/cmmm/2022/6312100/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human Ang-II concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

45218014 1.0 µg DNA

Spata19 ORF Vector (Mouse) (pORF)

Ask

View Details

MBS8120676-01 0.1 mg

CEACAM5 Protein, Human, Recombinant (hFc Tag)

Ask

View Details

MBS8120676-02 1 mg

CEACAM5 Protein, Human, Recombinant (hFc Tag)

Ask

View Details

MBS8120676-03 5x 1 mg

CEACAM5 Protein, Human, Recombinant (hFc Tag)

Ask

View Details

MBS6290525-01 0.1 mL

CYP2S1, CT (CYP2S1, Cytochrome P450 2S1, CYPIIS1) (MaxLight 550)

Ask

View Details

MBS6290525-02 5x 0.1 mL

CYP2S1, CT (CYP2S1, Cytochrome P450 2S1, CYPIIS1) (MaxLight 550)

Ask

View Details