Human IL-1β (Interleukin 1 Beta) ELISA Kit

Product Specifications

Abbreviation

IL-1β

UniProt

P01584

Cross Reactivity

This kit recognizes Human IL-1β in samples. No significant cross-reactivity or interference between Human IL-1β and analogues was observed

Target

IL1B; IL1-BETA; IL1F2; catabolin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1β. You can calculate the concentration of Human IL-1β in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S009286742031000X

Citation 02

https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.31056

Citation 03

https://www.nature.com/articles/s41380-020-0662-3.pdf?origin=ppub

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12855

Citation 05

https://link.springer.com/article/10.1186/s12951-022-01288-x

Citation 06

https://www.sciencedirect.com/science/article/pii/S0269749122014312

Citation 07

https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02535-6

Citation 08

https://www.sciencedirect.com/science/article/pii/S221112472030975X

Citation 09

https://www.nature.com/articles/s41419-020-03317-9

Citation 10

https://www.nature.com/articles/s12276-022-00729-9

Citation 11

https://www.nature.com/articles/s12276-019-0310-7

Citation 12

https://www.sciencedirect.com/science/article/pii/S0304383522000040

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IL-1β concentrations in serum, plasma and other biological fluids.

Available Sizes

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