Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit

Product Specifications

Abbreviation

VEGF-A

UniProt

P15692

Cross Reactivity

This kit recognizes Human VEGF-A in samples. No significant cross-reactivity or interference between Human VEGF-A and analogues was observed

Target

VEGF-A; VEGFA; MVCD1; VPF

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF-A. You can calculate the concentration of Human VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0142961222003957

Citation 02

https://www.sciencedirect.com/science/article/pii/S1385894722011925

Citation 03

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01461-2

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13316

Citation 05

https://clincancerres.aacrjournals.org/content/25/6/1957.abstract

Citation 06

https://www.nature.com/articles/s41419-022-04734-8

Citation 07

https://www.sciencedirect.com/science/article/pii/S0141813022022462

Citation 08

https://www.sciencedirect.com/science/article/pii/S0304383520304444

Citation 09

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-02739-w

Citation 10

https://www.mdpi.com/2072-6694/14/14/3365

Citation 11

https://www.sciencedirect.com/science/article/pii/S1742706119303277

Citation 12

https://www.sciencedirect.com/science/article/pii/S0378517322011206

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human VEGF-A concentrations in serum, plasma and other biological fluids.

Available Sizes

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