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Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Product Specifications

Abbreviation

TNF-α

UniProt

P01375

Cross Reactivity

This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed

Target

DIF; TNF-alpha; TNFA; TNFSF2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Immunology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNF-α. You can calculate the concentration of Human TNF-α in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Product Datasheet

https://789.bio/ea/huxz5g

Product MSDS

https://789.bio/eb/uj1O0G

Citation 01

https://www.sciencedirect.com/science/article/pii/S009286742031000X

Citation 02

https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.31056

Citation 03

https://pubs.acs.org/doi/abs/10.1021/acsnano.6b06658

Citation 04

https://jeccr.biomedcentral.com/articles/10.1186/s13046-020-01752-2

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/

Citation 06

https://academic.oup.com/ecco-jcc/advance-article-abstract/doi/10.1093/ecco-jcc/jjac171/6794137

Citation 07

https://link.springer.com/article/10.1186/s12951-022-01288-x

Citation 08

https://www.sciencedirect.com/science/article/pii/S0269749122014312

Citation 09

https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02535-6

Citation 10

https://pubs.acs.org/doi/abs/10.1021/acsami.9b04827

Citation 11

https://www.nature.com/articles/s41419-021-03777-7

Citation 12

https://www.sciencedirect.com/science/article/pii/S0304383522000040

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human TNF-α concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

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