Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit

Product Specifications

Abbreviation

IGF-1

UniProt

P05019

Cross Reactivity

This kit recognizes Human IGF-1 in samples. No significant cross-reactivity or interference between Human IGF-1 and analogues was observed

Target

IGF1; IGFI; IGF-I; IGF1A; IGF-IA; IGF-IB; MGF; Somatomedin C

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGF-1. You can calculate the concentration of Human IGF-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0304383521003323

Citation 02

https://www.hindawi.com/journals/sci/2021/5553676/

Citation 03

https://www.nature.com/articles/s41419-019-1670-6

Citation 04

https://link.springer.com/article/10.1007/s10803-020-04820-w

Citation 05

https://www.nature.com/articles/s41598-021-87583-3

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7502406/

Citation 07

https://jme.bioscientifica.com/view/journals/jme/aop/jme-22-0193/jme-22-0193.xml

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467354/

Citation 09

https://www.sciencedirect.com/science/article/pii/S0168822719302177

Citation 10

https://www.sciencedirect.com/science/article/pii/S0168822719302050

Citation 11

https://www.sciencedirect.com/science/article/pii/S030439402200132X

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218643/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IGF-1 concentrations in serum, plasma and other biological fluids.

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