Human IFN-β (Interferon Beta) ELISA Kit

Product Specifications

Abbreviation

IFN-β

UniProt

P01574

Cross Reactivity

This kit recognizes Human IFN-β in samples. No significant cross-reactivity or interference between Human IFN-β and analogues was observed

Target

IFNB1; IFB; IFF; IFNB

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-β. You can calculate the concentration of Human IFN-β in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41392-021-00703-3

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/

Citation 03

https://www.sciencedirect.com/science/article/pii/S1521661623000177

Citation 04

https://www.nature.com/articles/s41388-021-01869-4

Citation 05

https://www.nature.com/articles/s41419-020-2665-z

Citation 06

https://www.sciencedirect.com/science/article/pii/S2211124719300397

Citation 07

https://link.springer.com/article/10.1186/s13578-021-00687-1

Citation 08

https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009366

Citation 09

https://www.nature.com/articles/s41419-020-2625-7

Citation 10

https://www.frontiersin.org/articles/10.3389/fimmu.2017.01051/full

Citation 11

https://www.frontiersin.org/articles/10.3389/fimmu.2017.00779/full

Citation 12

https://www.mdpi.com/1090868

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IFN-β concentrations in serum, plasma and other biological fluids.

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