Human SDF-1/CXCL12 (Stromal Cell Derived Factor 1) ELISA Kit

Product Specifications

Abbreviation

SDF-1; CXCL12

UniProt

P48061

Cross Reactivity

This kit recognizes Human SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Human SDF-1/CXCL12 and analogues was observed

Target

SDF1; CXCL12; IRH; PBSF; SCYB12; TLSF; TPAR1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology; Microbiology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SDF-1/CXCL12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDF-1/CXCL12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SDF-1/CXCL12. You can calculate the concentration of Human SDF-1/CXCL12 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/

Citation 02

https://www.nature.com/articles/s41419-020-2665-z

Citation 03

https://www.sciencedirect.com/science/article/pii/S1742706118302460

Citation 04

https://www.sciencedirect.com/science/article/pii/S1742706121004128

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037856/

Citation 06

https://www.mdpi.com/article/10.3390/pharmaceutics14091803

Citation 07

https://www.sciencedirect.com/science/article/pii/S0753332218372512

Citation 08

https://academic.oup.com/ndt/advance-article-abstract/doi/10.1093/ndt/gfaa335/6067632

Citation 09

https://ec.bioscientifica.com/view/journals/ec/aop/ec-21-0629/ec-21-0629.xml

Citation 10

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0188007

Citation 11

https://www.jstage.jst.go.jp/article/endocrj/advpub/0/advpub_EJ21-0039/_article/-char/ja/

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1111/1744-9987.13633

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids.

Available Sizes

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