Human CRP (C-Reactive Protein) ELISA Kit

Product Specifications

Abbreviation

CRP

UniProt

P02741

Cross Reactivity

This kit recognizes Human CRP in samples. No significant cross-reactivity or interference between Human CRP and analogues was observed

Target

PTX1; Pentraxin 1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.39-25 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.23 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2041788

Citation 02

https://pubmed.ncbi.nlm.nih.gov/32949690/

Citation 03

https://www.sciencedirect.com/science/article/pii/S0003267022007000

Citation 04

https://www.mdpi.com/2227-9059/9/12/1931

Citation 05

https://academic.oup.com/ndt/advance-article-abstract/doi/10.1093/ndt/gfaa335/6067632

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517782/

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcpe.13400

Citation 08

https://www.sciencedirect.com/science/article/pii/S0022395622001972

Citation 09

https://www.mdpi.com/2072-6643/12/9/2644

Citation 10

https://www.nature.com/articles/s41598-021-87583-3

Citation 11

https://www.sciencedirect.com/science/article/pii/S0899900721001684

Citation 12

https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/iub.1458

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in serum, plasma and other biological fluids.

Available Sizes

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