Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit

Product Specifications

Abbreviation

BDNF

UniProt

P23560

Cross Reactivity

This kit recognizes Human BDNF in samples. No significant cross-reactivity or interference between Human BDNF and analogues was observed

Target

ANON2; BULN2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BDNF. You can calculate the concentration of Human BDNF in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41419-022-05068-1

Citation 02

https://www.nature.com/articles/s41419-019-1670-6

Citation 03

https://www.mdpi.com/978204

Citation 04

https://www.sciencedirect.com/science/article/pii/S1382668922001727

Citation 05

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03896f

Citation 06

https://www.sciencedirect.com/science/article/pii/S1756464622002900

Citation 07

https://www.mdpi.com/2013440

Citation 08

https://www.sciencedirect.com/science/article/pii/S0022395622001972

Citation 09

https://www.sciencedirect.com/science/article/pii/S0924977X22001286

Citation 10

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0731-y

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8506992/

Citation 12

https://www.sciencedirect.com/science/article/pii/S0920996418301166

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human BDNF concentrations in serum, plasma and other biological fluids.

Available Sizes

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