4-HNE (4-Hydroxynonenal) ELISA Kit

Product Specifications

Abbreviation

4-HNE

Cross Reactivity

This kit recognizes Universal 4-HNE in samples.No significant cross-reactivity or interference between Universal 4-HNE and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Neuroscience; Cell Biology; Metabolism

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 4-HNE. During the reaction, Universal 4-HNE in the sample or standard competes with a fixed amount of Universal 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.63-40 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.38 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1385894722044709

Citation 02

https://www.sciencedirect.com/science/article/pii/S014296122200360X

Citation 03

https://www.sciencedirect.com/science/article/pii/S2213231721002895

Citation 04

https://www.sciencedirect.com/science/article/pii/S0144861722002302

Citation 05

https://www.sciencedirect.com/science/article/pii/S0891584922005822

Citation 06

https://www.mdpi.com/2163138

Citation 07

https://www.hindawi.com/journals/omcl/2023/8258354/

Citation 08

https://www.sciencedirect.com/science/article/pii/S089158492200020X

Citation 09

https://www.sciencedirect.com/science/article/pii/S0753332222010204

Citation 10

https://www.sciencedirect.com/science/article/pii/S0048969720329429

Citation 11

https://www.mdpi.com/1576784

Citation 12

https://www.mdpi.com/1033142

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of 4-HNE concentrations in serum, plasma and other biological fluids.

Available Sizes

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