A-CoA (Acetyl Coenzyme A) ELISA Kit

Product Specifications

Abbreviation

A-CoA

Cross Reactivity

This kit recognizes Universal A-CoA in samples.No significant cross-reactivity or interference between Universal A-CoA and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Tags and Cell Markers; Cardiovascular; Metabolism

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Universal A-CoA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Universal A-CoA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Universal A-CoA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Universal A-CoA. You can calculate the concentration of Universal A-CoA in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0142961222005427

Citation 02

https://www.embopress.org/doi/abs/10.15252/embj.2021110218

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/jipb.13283

Citation 04

https://www.sciencedirect.com/science/article/pii/S002432052300125X

Citation 05

https://www.mdpi.com/1999-4923/14/9/1759

Citation 06

https://www.sciencedirect.com/science/article/pii/S0006295222002933

Citation 07

https://www.hindawi.com/journals/omcl/2020/5849794/

Citation 08

https://www.sciencedirect.com/science/article/pii/S0304416523000016

Citation 09

https://www.sciencedirect.com/science/article/pii/S0303720718300789

Citation 10

https://link.springer.com/article/10.1007/s40200-022-01135-8

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of A-CoA concentrations in serum, plasma and other biological fluids.

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