FT4 (Free Thyroxine) ELISA Kit

Product Specifications

Abbreviation

FT4

Cross Reactivity

This kit recognizes Universal fT4 in samples.No significant cross-reactivity or interference between Universal fT4 and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signaling transduction; Developmental Biology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal fT4. During the reaction, Universal fT4 in the sample or standard competes with a fixed amount of Universal fT4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal fT4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal fT4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0269749121009040

Citation 02

https://www.sciencedirect.com/science/article/pii/S004896972100262X

Citation 03

https://www.mdpi.com/1422-0067/23/15/8244

Citation 04

https://www.sciencedirect.com/science/article/pii/S2001037020304761

Citation 05

https://www.hindawi.com/journals/omcl/2021/2482453/

Citation 06

https://www.sciencedirect.com/science/article/pii/S0304389418300050

Citation 07

https://www.sciencedirect.com/science/article/pii/S0147651319301538

Citation 08

https://www.sciencedirect.com/science/article/pii/S0269749119349905

Citation 09

https://jme.bioscientifica.com/view/journals/jme/67/3/JME-21-0095.xml

Citation 10

https://www.nature.com/articles/s41598-021-00903-5

Citation 11

https://www.sciencedirect.com/science/article/pii/S0166432822003503

Citation 12

https://www.sciencedirect.com/science/article/pii/S0045653515007572

Detection Instrument

Microplate reader

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of fT4 concentrations in serum, plasma and other biological fluids.

Available Sizes

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